ABSTRACT
Evaluation of the toxic effects of synthetic dyes brilliant blue were tested in rats by measuring their actions on serum activity of glutamate oxaloacetate transaminas [AST], glutamate pyruvate transaminase [ALT], alkaline phosphatase [ALP], acid phosphatase [ACP], serum total bilirubin [SBIL-T], serum creatinine [SCR], serum urea [SUR] and serum testosterone concentrations. Rats were fed synthetic brilliant blue dye supplemented diet, daily for 15, 30 and 45 days. Brilliant blue dye caused an increase of ALT, AST, ALP, SBIL-T, SUR and SCR. This increase was more pronounced in animals treated with repeated single higher doses than in those receiving the repeated single lower doses. On the contrary, serum ACP and testosterone concentrations were decreased after treatment. Histopathological examinations revealed alterations in kidneys include: congestion and hemorrhage with infiltration, thick walled blood vessels and deformation of the structure of glomeruli. Whereas alterations in liver include: focal necrosis of hepatocytes, infiltration and vacuolation. Testes showed irregular shape of seminiferous tubules, atrophy of Leydig cells and disturbance in spermatogenesis. Results indicated that the used doses of the synthetic dye brilliant blue were mostly attributable to hepatocellular damage, renal failure and decrease in spermatogenesis process
Subject(s)
Animals, Laboratory , Male , Rats , Spermatogenesis/drug effects , Liver Function Tests/drug effects , Kidney Function Tests/drug effects , Testis/drug effects , Hepatocytes/drug effects , Food Coloring Agents/toxicity , Food Coloring Agents/adverse effectsABSTRACT
Demostramos anteriormente que la solubilidad del colorante alimentario indigotina se modifica en el hígado de mamíferos, volviéndose totalmente insoluble en solventes acuosos y altamente soluble en solventes orgánicos. En el presente trabajo se estudió la movilidad cromatográfica de la indigotina presente en un extracto lipídico de hígado de rata, 20 minutos después de su administración intravenosa. Se estudió además la presencia de grupos funcionales correspondientes a su probable conjugación con fosfolípidos, en el extracto preparado al raspar la banda del colorante separada mediante cromatografía de fosfolípidos en capa delgada. Se detectaron 0,144 +/- 0,087 µg de fósforo fosfolipídico y se demostró la presencia de ácidos grasos en dicha fracción, proveniente de un extracto preparado a partir de 2 gramos de hígado. Estos resultados confirman el aumento en la solubilidad del colorante en solventes orgánicos una vez que ha ingresado al hígado y sugieren que el mismo sufre una probable conjugación con fosfolípidos en dicho órgano.
We have demonstrated that the solubility of the food colorant indigotin was modified in mammalian liver, where it becomes totally insoluble in aqueous solvents and highly soluble in organic ones. This work presents the results from the studies on the chromatographic mobility of indigotin present in the lipidic extract obtained from rat liver 20 minutes after its intravenously administration. The presence of functional groups corresponding to its probable conjugation with phospholipids was also studied in the extract prepared from the colorant spot separated by thin layer chromatography of phospholipids. 0,144 +/- 0,087 _g of phospholipidic phosphorus were detected and fatty acid presence was demosntrated in this fraction, obtained from liver extract prepared with 2 grams of tissue. These results confirm the increment of the colorant solubility in organic solvents once it is incorporated in the liver and they suggest that it suffers a probably conjugation with phospholipids in this organ.
Subject(s)
Animals , Food Coloring Agents/analysis , Food Coloring Agents/toxicity , Liver/metabolism , Phospholipids , Food Additives/analysis , Food Additives/toxicity , Food Industry/methods , Rats, WistarABSTRACT
In last few years, all over the world, food preservatives and favorable food colour are used in wide scale. However, their use in food still controversial. It causes and will cause severe tension to the consumers as the sensitivity of people to general health increases. This work was carried out to study the possible toxic effect of the interaction of one of food preservatives [sod. nitrite] and one of the most favorable food colour [sun-set yellow] on rates. To study the effect of this interaction, a mixture of 1/10 of the limited dose of Na No3 and sun-set yellow was daily administered to rats. Other group was supplemented with royal jelly in combination with the mixture to evaluate its possible protective role during the course of experiment. Treatments were continued to 30 days, then half of the animals were sacrificed, the other half was left for 15 days after the last dose without any additional treatment [as a recovery period]. The result can be summarized as follows: 1. Administration of sod. nitrite and sun-set yellow produced a significant decrease in percentage of body weigh, W.B.Cs, R.B.Cs, Hb, Hct, inorganic phosphorus, serum protein and serum albumin of rats. 2. A marked increase in respiration rate, serum glucose, T3, T4, calcium, gamma-GT, LDH, CPK, alk. ph., serum cholesterol and [brain, liver and heart cholesterol] was recorded during treatment with the mixture. 3. Insignificant change in organ / b.wt., heart beat, rectal temperature, serum and tissue AST and ALT, serum acid phosphates, tissue proteins, serum and tissue total lipids muscle and kidney cholesterol and serum triglycerides was determined. 4. Administration of royal jelly and to some extent a recovery period ameliorated many hazards produced by using food additives. So, this study threw light on the bad behavior and its hazards of using food additives and food colour in the same time by our kids. It is also clear that royal jelly as a natural product nearly ameliorate these damage. So, it's advisable to administered royal jelly to children and prevent if possible the using of additive and synthetic colour in their food
Subject(s)
Animals, Laboratory , Food Coloring Agents/toxicity , Food Preservatives , Rats , Respiratory Rate , Food Additives , Blood Glucose , Fatty Acids , Protective AgentsABSTRACT
The dose-dependent effect of mentanil yellow (MY) on the development of preneoplastic hepatic lesions during N-nitrosodiethylamine (DEN) induced hepatocarcinogenesis was studied in comparison with phenobarbitone (PB), in male Wistar (WR) rats. Rats were administered 200 ppm DEN through drinking water for a period of 1 month. After an interval of 2 wk, the animals were administered MY at concentrations of 0.1, 0.5 and 1.0 per cent in the diet for a period of 7 months. PB at 500 ppm served as the standard tumour promoter. The dose-dependent tumour promoter effects of MY were monitored on the basis of morphological appearance of the livers, liver weight profile, histological pattern, appearance of gamma-glutamyl transpeptidase (GGT) positive foci, total GGT activity and the induction of glycogen-deficient islands. All the three doses of MY were found to enhance liver carcinogenesis when compared with either the corresponding controls or only the DEN treated animals. MY at 0.1 per cent was found to be more effective as an enhancer of DEN-induced carcinogenesis than 0.5 and 1.0 per cent. In the present study a dose-related enhancing effect of MY on DEN-induced hepatic preneoplasia in rats has been demonstrated.